![]() Rapid detection of ethambutol-resistant Mycobacterium tuberculosis strains by PCR-RFLP targeting embB codons 306 and 497 and iniA codon 501 mutations. Molecular fingerprinting of isoniazid-resistant Mycobacterium tuberculosis isolates from chest diseases hospital in Kuwait. History and principles of conductive media for standard DNA electrophoresis. Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media. Yuryev, A.) (Humana Press, Totowa, NJ, 2007).īrody, J.R., Calhoun, E.S., Gallmeier, E., Creavalle, T.D. The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Preparation of genomic DNA from mammalian tissue. Single-step PCR optimization using touchdown and stepdown PCR programming. Type-specific amplification of viral DNA using touchdown and hot start PCR. Molecular identification and immunolocalization of the water channel protein aquaporin 1 in CBCECs. Combining restriction digestion and touchdown PCR permits detection of trace isoforms of histamine H3 receptor. Analysis of circadian liver gene expression by ADDER, a highly sensitive method for the display of differentially expressed mRNAs. Kornmann, B., Preitner, N., Rifat, D., Fleury-Olela, F. Mapping transposon insertion sites by touchdown PCR and hybrid degenerate primers. Use of degenerate primers and touchdown PCR to amplify a halogenase gene fragment from Streptomyces venezuelae ISP5230. Use of degenerate primers and touchdown PCR for construction of cDNA libraries. Comparison of single and touchdown PCR protocols for detecting Pneumocystis jirovecii DNA in paraffin-embedded lung tissue samples. The use of 'touchdown' polymerase chain reaction increases the sensitivity and specificity of t(11 14)(q13 q32) detection in patients with mantle cell lymphoma. Multistep-touchdown vectorette-PCR-a rapid technique for the identification of IVS in genes. Single-tube heminested PCR coupled with 'touchdown' PCR for the analysis of the walleye dermal sarcoma virus env gene. High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR. ![]() Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes. Improved nearest-neighbor parameters for predicting DNA duplex stability. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction. Stability of ribonucleic acid double-stranded helices. OligoCalc: an online oligonucleotide properties calculator. Comparison of different melting temperature calculation methods for short DNA sequences. Quantification of mRNA using real-time RT-PCR. Touchdown thermocycling program enables a robust single nucleotide polymorphism typing method based on allele-specific real-time polymerase chain reaction. 'Touchdown' PCR to circumvent spurious priming during gene amplification. Enzymatic amplification of DNA by PCR: standard procedures and optimization. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. ![]()
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![]() The first round of grants are expected to be awarded in September 2023. The Downie & Wenjack Fund will begin accepting applications for Oshki Wupoowane - The Blanket Fund early next year. There's still so much work to do.… We can't just wear orange shirts on the one day and leave it at that," she said.īut "steps towards recognizing the truth of what happened in the past and the truth of how that carries forward today … still progress, and progress is good." "There will be critics and I think there's still things you can criticize the company, and a lot of other institutions in Canada, for. It's an important gesture by a corporate leader, she said. (Tyson Koschik/CBC)įay said she was happy to hear about the launch of Oshki Wupoowane, saying it shows HBC acknowledges its role in colonialism. For a king, you'd need a four-stitch blanket. For example, two stitches will fit a twin-sized bed. The stitches of indigo-dyed wool indicate the size of the blanket. Ian Pace : Gordon, you seem as committed an advocate of the ideals of 'high' modernism in music (as well as in architecture and other artistic media) as just about any composer I have encountered. Many Canadians associate the multicoloured stripes of the blankets "as synonymous with HBC and synonymous with … a very romanticized history of the fur trade," said Fay.Īn iconic HBC point blanket at the Manitoba Museum. "The point blankets and the stripes themselves ended up becoming a rather iconic symbol for the company," she said. The HBC point blanket has been many things in its history - a valuable trade item, an emblem of Canada rumoured as a carrier of disease, and a symbol of colonialism, says Amelia Fay, curator of the Hudson's Bay Company Museum collection at the Manitoba Museum. "We also need to start doing the work, and this is one way that we can move forward together." A complicated symbol 'Historic and monumental' step taken as Winnipeg's Bay building handed to First Nations groupĪcknowledging the past is important, she said, "but ultimately we need to look towards the future and ensuring the strength and resilience and vibrancy of Indigenous peoples and communities for the next seven generations.".Analysis Hudson's Bay Company has 350 years of history - but does it have a future? ![]() ![]() ![]() You may like to set up an alert for your favourite authors or subjects. Due to volume of requests we are unable to respond individually so please keep an eye on The Library Catalogue to see any new additions. Members are welcome to make recommendations. The Librarian Team review suggestions monthly. Through JSTOR you can access a huge digital library of academic journals, books, primary sources and periodicals. Read a good book and think the library should have a copy? Let us know! Journal article requests form Make a suggestion These cookies are used for things like showing relevant adverts based on website visits. Please click on the links below to access this service: These services may use cookies to help make advertising more effective. Among the online historical diaries available include the handwritten journal of the only member of the Lewis and Clark expedition to die en route, Sgt. This includes cases where providers may have the item but are not loaning due to the Covid-19 pandemic. This is non-refundable, even if the item cannot be supplied and needs to be paid at the time of ordering. Please be aware that there is a £5.00 charge for this service per item. Just print out the PDF template I’ve created, trim to size, fold along lines and glue to your project. To request an item that is not held by Gloucestershire Libraries you can request an ‘inter library loan" (ILL) and a search will be made to see if it can be borrowed from another library. You can probably find them in old library books or vintage paper lots, but it’s a lot easier to just make your own Use my printable library card template to make as many as you need for your cards, journals and planner decoration. Ordering items from other libraries (Inter Library Loans/ILLs) I upcycle a thrift store book, walk you through a quick kettle stitch binding tutorial, an. If you do not have an email address registered on your account or have any queries please Contact the Library. This is a tutorial on how to make a junk journal from an old book. If you have forgotten your PIN and have an email address registered on your account you can re-set your PIN online. Vintage book letters, Old books, Readers digest books, letters made from books, cut book letters, book cut into alphabet, great for bookends. You will need your library number and PIN number to access your account. ![]() New features on The Library Catalogue - July 2022 Make an Appointment Newspapers Journals Collections Program Borrow & Renew Library Search Find books, audio and video, music scores, maps and more in. Log in to your account to renew your books and view your reserved items. You can reserve library books, Reading Group Collections and other items for collection from any Gloucestershire Library. Use the online catalogue to browse the collection. ![]() ![]() ![]() One major issue is there's a chance that it may not detect the correct save format or will corrupt the existing save file so make sure to backup your saves beforehand (they are currently trying fix it by having a better database for save detection, if you're interested you can help). It can load the ROM through the SD card instead of having to convert and install them, but needs to be launched on boot instead of being able to use the 3DS HOME Menu (there's ways to make a forwarder for in on the HOME menu, but requires additional setup to get it to work). The major advantages of this compared to VC injection is that it performs faster and there aren't any issues with the sound, it utilizes the native hardware (even with VC injection there are issues with the sound, ex.: "Fire Emblem").
![]() ![]() “We know that affordability is a major barrier to education, so we hope that by doubling our funding, we can double the number of doors opened for young people, and remove twice as many obstacles for innovators, creators and dreamers. “When we first committed to awarding $10 million, we never imagined we would be more than doubling it so quickly,” said Tina Nguyen, executive director of the Taco Bell Foundation. The launch came amid national attention on education access-during American Education Week and National Scholarship Month-to bolster awareness about the opportunities available to students. Just a few weeks ago, Live Ms Scholarship recipients applied their passions and talents during the Taco Bell Skills Challenge at the NBA All-Star game from creating social media content to photographing the event, designing the trophy and appearing in our ad. The Taco Bell Foundation is also opening its Live Más Scholarship application for the fourth consecutive year, with more than $4 million on the table for passionate young students, $1 million more than what was awarded this past year. We did a lot of amazing things together in 2016, and we’ve already kicked off 2017 strong. Application Requirements : Submit an original video. 12, 2018, that it is ahead of schedule to meet its commitment to award $10 million in Live Más Scholarships by 2022, and more than doubled that commitment to a total of $21 million by 2021. WitrynaThe Live Ms Scholarship is available for both Taco Bell restaurant employees and non-employees. It could be a short film, animation, or a simple testimonial.The Taco Bell Foundation announced on Nov. Think about filming this video on a phone, computer, tablet, or any other MP4 video-enabled camera to which you have access. Criteria: Applicant is a legal resident of the 50 United States and. Your submission video must be between 30 seconds and 2 minutes in length. Live Mas Scholarship from the Taco Bell Foundation. How will your education help enable you to pursue your passion and make a change.How do you plan to use your passion to uniquely make a positive change in your community or the world?.The scholarship amount varies from 5,000 to 25,000, depending on the level of education and financial need. What are the Benefits The Taco Bell Scholarship provides financial assistance to students pursuing their educational dreams. What is your passion and how are you currently pursuing it? The Live Ms Scholarship is for students who are pursuing higher education, preparing for the workforce, or using their passion to ignite change in their communities and beyond. The application deadline is usually in January, so make sure to submit your application before the deadline.Submit a video (2 minutes or less in length) that addresses these questions: and its parent, subsidiaries of its parent, franchisees, licensees, and the advertising and promotion agencies involved with this Program are not eligible to apply or receive a scholarship through this version of the program. Employees of Taco Bell Foundation, Inc., Taco Bell of America, LLC, Taco Bell Corp.Applicants have no obligation to affiliate with or. Important Dates: Deadline is J(Apply soon) Number of Recipients: 100 high school juniors (with the option to renew their senior year). Applicant must be willing to work with the Taco Bell Foundation’s Scholarship Management Company throughout the application and award process. Final deadlines vary by school, but the early application deadline for most scholarships is June 1, 2023.Applicant must be on-track to apply for or enrolled in an accredited post-high school/post-secondary educational program (including accredited two-and four-year colleges, universities, vocational-technical and trade schools).If an applicant is a minor in his/her/their state of residence, they must receive parental consent from his/her/their parent/legal guardian to apply.Applicant is a legal resident of the 50 United States and the District of Columbia (“D.C.”) who is at least 16 years of age and no older than 26 years of age as of the last day of the Program Period located in the United States or D.C.EligibilityĪll applicants must meet all of the following criteria to be considered eligible for the 2023 Live Más Scholarship Program: Since 1992, the Taco Bell Foundation has reached more than 4 million young people across the country and has awarded more than $110 million in Live Más Scholarships and grants to youth-serving nonprofit organizations focused on education and career readiness. The Taco Bell Foundation breaks down barriers to educate and inspire the next generation of America’s leaders. ![]() ![]() ![]() ![]() Please see below where the closest amenities can be found. You can stay close to the villa and enjoy the calmness of the area, the panoramic views and one of the best beaches in Crete or you can take advantage of the villas central location and explore our amazing island! In the village of Agia Triada you will only find a small traditional tavern that you will love! Moreover the bigger villages "Pigi" and "Adele" where you can find all kind of shops, supermarkets, bakeries and several taverns are only 3 km away! About 6 km away from the villa you will also find the well known tourist area of "Platanias" with many organised beaches, night clubs, big market and restaurants! Given the location there are also numerous attractive hiking and cycling routes. INFORMATION ABOUT THE AREA Villa Hara is located on a hill next to "Agia Triada" village, a small village with quietness which is ideal for relaxing, and for almost all kind of vacations. Parking space for 2 cars outside the Villa. A spacious garden with area for kids to play and a playground spot next to the pool. ?utdoor sitting ad dining areas next to the pool under the shade of a pergola. We will also provide you with everything that you might require such as charcoal. In the garden there is a built-in barbecue facility and a seating area under a big tree where you can enjoy a meal or drink under the shadow. The pool area is equipped with sun beds, side tables, a poolside shower and umbrellas. The outdoor facilities include: - A private, 20 m? private swimming pool, 1,60 m. Its the perfect choice for travellers who value quality, privacy and the experience of a true holiday. The Villa combines an elegant natural setting with full privacy, allowing to completely unwind and relax. Outdoor Area Set on a private field of 4.000 meters, the exterior of Villa Hara enjoys breathtaking views over the endless deep blue of the sea and sky, but even from the balcony you may have views of the whole city. Finally, the studio offers direct access to the pool terrace. Additionally, the studio features its own lounge with fireplace, as well as a kitchenette. The autonomous studio on the first floor has its own private entrance and hosts the fourth bedroom with a double bed (which can also can be set up as two single beds upon request) and en-suite bathroom with shower cabin. One of the bedrooms enjoys an en-suite bathroom with a hydromassage cabin, while the remaining two bedrooms share a bathroom with bathtub, a washer/dryer and hair dryer. Each of the 3 ground floor bedrooms offers a double bed, individually controlled A/C, closet & direct access to the garden. ![]() The kitchen is fully equipped with kitchenware and home appliances such as dishwasher, microwave, toaster and coffee machine, as well as a dining table. ![]() The living area boasts an open fireplace, 55 smart TV, as well as direct access to the garden. The main house on the ground level features the living room, kitchen, 3 bedrooms and 2 bathrooms. separated studio- and can accommodate 8 people in beds and up to 9 people if needed in its 4 bedrooms. Built in 2006 and renovated in 2012, the property consists of 2 buildings in two different levels -a 120 sq.m. Layout Villa Hara brings a traditional home feeling to your holidays, with its Cretan character, decorated in a rustic manner with traditional furniture. Given the location of the village and the landscape, there are numerous attractive hiking and cycling routes. The small village of Agia Triada is appointed in the narrowest point of Crete, which gives you easy access (within 40 minutes) to the most beautiful Southern beaches of Rethymno, making it an ideal holiday base for your trips and excursions and is located just 5,5 Km away from the closest beach. In 1-1,5 km you can find a mini market, pharmacy, 3 tavernas and a butcher. Set in an area of magnificent natural beauty, surrounded by gardens of pretty flowering shrubs and fruit trees, Villa Hara is also conveniently located close to shops for your everyday needs. Built on an elevated spot at the village of Agia Triada, just 9Km away from the renaissance city of Rethymno, Villa Hara offers breathtaking views of the sea and Psiloritis Mountain. Villa Hara is approved by Greek Tourism Organization with license number: 1041K92003023201 & managed by "etouri vacation rental management". ![]() ![]() See our fine collection of segmental block retaining walls. It is also easy to create custom design that will suit your needs and preferences. To ensure that you get colours that match, its a good idea to get your wall stones, curbs and edgers, and pavers from the same manufacturer. innovative, universal, versatile and user-friendly. The Pro-Line series of adjustable pedestals from Eurotec, Inc. These innovative features will allow you or your contractor to complete the installation at a faster rate. Eurotec 7.4803-in W x 0.3937-in H x 7.4803-in L Deck Concrete Block. Portland Rock & Landscape Supply carries pavers from multiple suppliers, including Keystone, Mutual Materials, Castohn, and MSI. The segmental type is flexible and easy to install because of its interlocking block units. Sunroc is a leading manufacturer and supplier of masonry block, segmental retaining walls, pavers, and fence block. These interlocking, precast concrete or porcelain pieces come in a variety of sizes, shapes, and colors, making them versatile and easy to use for different landscaping designs. You can even build up to 50 feet walls without needing structural reinforcements. Our high-quality retaining walls and pavers include a range of materials such as concrete blocks, natural stone, and clay pavers, sourced from trusted. The segmental retaining wall blocks are made from the finest raw materials, which make them extremely durable masonry products. The latter is gaining popularity because of its excellent features, aesthetic appeal, and durability. SOUTH FLORIDAS TOP SUPPLIER OF NATURAL STONE Operating out of South Florida, Epic Stoneworks is the best supplier for paving stone, coral stone veneer, block walls, and beach pebbles for all of your landscaping, architectural, and construction projects. Your options include natural stones, concrete blocks, and segmental wall blocks. ![]() There are different types of materials that can be used for your retaining walls. Why Choose Segmental Retaining Wall Blocks? We can help you build sturdy and stunning retaining walls by providing you with premium-quality retaining wall blocks from Belgard and other leading manufacturers. Get your products from trusted Michigan suppliers like us at Grahek's, Inc. Your flower gardens and other plantings will also look great if they are contained in well-designed retaining walls.īut before you start building any retaining wall in your landscape or property, make sure you have access to premium-quality wall blocks. ![]() You can also use the structures to create new functional outdoor spaces such as sitting areas, patios, and decks. ![]() They are not only built to prevent possible soil erosion in the outdoor area but also installed to improve the aesthetic appeal of your property. NEED HELP WITH DESIGN AND INSTALLATION GET AN ESTIMATE Block Landscaping Ideas Gallery The Brickyard has been producing & selling block in Western Colorado & Eastern Utah for over 30 years. ![]() Retaining walls are vital features in your property. NATURAL IMPRESSIONS-FLAGSTONE (main block 300X175X100H. Order online for delivery or Click & Collect at your nearest Bunnings. Melbourne Brick Melbournes leading retailer of bricks, blocks, pavers, retaining walls, synthetic grass, geohex, concrete sleepers & landscaping. Scroll to bottom of page for Wall Block Gallery.Īll images are for display purposes only, click on company logos below for more information Shop our range of Retaining Wall Blocks at warehouse prices from quality brands. We have a great range of blocks to suit all your needs with competitive pricing and a range of delivery options.Come visit our yard to view our numerous displays in person to get a better idea of what you are after. We are outdoor experts and providers of outdoor furniture, grills and outdoor ovens, concrete retaining wall systems, concrete pavers, natural landscape rock, and other landscaping and outdoor-living supplies for the Minneapolis and St. ![]() ![]() ![]() Thanks for all help, I'm very green when it comes to wiring and guitar restoration but would love to learn more and I figure this h802 is a good place to start. From the above wiring diagram it looks like it should go to the volume pot but I'm not certain which part, perhaps where the silver wiring initially connects from the pickup selector?Īs you can see the pick guard isn't looking too good, rather than replacing I'm just going to cut a piece of vinyl and attach it to make up for the broken portion. Based off of this wiring diagram, it looks like the wire in the clear housing coming off the input should be soldered to the tone pot? In this photo have placed said wire over where I'm assuming it should go, is this correct. I am seeking help determining where to solder them, here is what I'm working with. Got this Harmony H802 with two severed wires, one is coming from the input and the other is the grounding wire. Combine Guitars - unusual kits & customs.Seymour Duncan Wiring Diagrams For most of your wiring diagram needs, a pretty comprehensive guide.Official Luthiers Forum Lots of great stuff here, Mainly for acoustics, but also for electrics.Focuses on telecasters, has a lot of info on building and finishing (check the "Tele Home Depot" and "Finely Finished" subforums).talkbass The "Luthier's Corner" has lots of info, step by step tutorials and build logs (mostly basses, of course).Musical Instrument Makers Forum Lots of tutorials, sign up needed to see the forum archives, but highly recommended.Electric Herald Tutorials, guides, and other useful information. ![]() Project Guitar great resource, with many tutorials./r/CustomMadeInstruments - for everything else in custom instruments./r/obscureguitars - for everything weird, wonderful, and unusual in the luthier world./r/bass - for all your bass related needs./r/guitarplaying - post videos of your talent!.We have done a lot of research to find the top 20 Harmony Electric Guitar H802 available. /r/guitar - for all your guitar related needs Choosing the Harmony Electric Guitar H802 can be difficult as there are so many considerations, such as Harmony, Sawtooth, Monoprice, Fender, Gibson, Epiphone, Jackson, Yamaha, ESP, Gretsch, Music Man.Everything about making musical instruments Related Reddits ![]() ![]() ![]() Among these changes is a buffer switch, which engages a unity-gain Class-A buffer circuit on the Thru output, preventing any loading down of passive pickups when connected to an amplifier and preserving the natural tone of your instrument. ![]() The active DI portion of the Reamp Station is based off of Radial’s world-class J48, tweaked slightly to provide maximum benefit for recording tracks that can be Reamped later on. Reamping is often something that’s overlooked whilst recording, however it can be a lifesaver if your guitar tones aren’t quite what you’re looking for.Īs the name suggests, reamping is the act of capturing a both a clean D.I and your amped sound simultaneously, therefore enabling you to play it back through any guitar amp at a later date and re-recording that tone.The Reamp Station combines two studio essentials in one chassis: an active DI box for pristine recordings of any instrument, and the JCR high-performance Reamper to feed those recordings to your guitar amps and pedals during mixdown.Ī great Reamping session starts with a clean, high-quality direct recording -which is why the DI you choose is so important. If you’re not happy with the original tone you recorded, reamping essentially acts as undo (or redo) button meaning you’re able to keep the same performance but tweak the tone of the amp, audition different pedals and experiment with multiple microphones and placement. Reamping is especially useful if you’re recording in a non-studio setting. ![]() ![]() Most of the time you’ll find that an idea hits you in the middle of the night when you’re not able to plug into your noisy amp. Re-amping means you’re able to get the performance down and worry about tone later. If you don’t have an amp to record with or you’re not wanting to record with your own amp, then all you’ll need to do is plug into your interface and record a clean D.I track. However if you’re planning on recording your amp and reamping later then you’ll need to have a couple of pieces of equipment, the most important of those being a D.I box, reamp box and of course an audio interface. The D.I box is placed first in the signal chain because you need to ensure you capture a completely clean (free from fx or distortion) guitar signal, which you’ll then be able to send into an amp. So now you’ve recorded your clean D.I you’re ready to start reamping. #Reamp recording studios proįirst of all you’ll need to use your DAW to prepare your tracks, in this case we’re using Logic Pro X. On your audio interface send a balanced cable out of a spare output into your re-amp box and a guitar lead from your re-amp box to your amp. It is important to use a re-amp box because amps operate on a different impedance to what your audio interface is sending. Set up a microphone as if you were to record a cab normally. Send your clean D.I out of a spare output on your interface. Tip: Using your DAW’s I/O plugin means that when recording your reamped signal you won’t hear back the clean D.I through your monitors. Hitting the ‘Ping’ button will send a short click through your amp, which is used to adjust for latency. Set up a new audio track for your mic channel and hit play, you should now hear your D.I through your amp.Īdjust your amp settings as normal and record the guitar track onto the new channel.Īs reamping isn’t the most common technique in recording, it requires a bit more equipment and initial investment than recording guitars in the traditional way. However, if you’re looking for an interface with the ability to reamp (and lots more features) then look no further.įeaturing two Audient console mic pres, Two notes power and and cab emulation, on board 12AX7 valve preamp and a re-amp output, Sono is the perfect audio interface for guitarist and engineers alike. Whether you’re looking to record your guitar, just practise or even play live then Sono is the audio interface for you. In the case of reamping, we could do away with the D.I box and re-amp box as Sono has a few tricks up its sleeve which provides the same functionality in one box. Once you’re ready to reamp, all you need to do is follow the same instructions above but instead of connecting the output of your interface to a re-amp box, you’ll just need to plug a guitar lead from the re-amp output of Sono (output 3) and into your amp – it’s as easy as that! Instead of having to connect a D.I box to your signal chain, Sono has the ability to automatically record a clean D.I signal as well as a processed version with the Two notes cab simulation simultaneously. ![]() ![]() ![]()
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